Purification and Assay of Rubisco Activase from Leaves

Ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) activase protein was purified from spinach leaves by ammonium sulfate precipitation and ion exchange fast protein liquid chromatography. This resulted in 48-fold purification with 70% recovery of activity and yielded up to 18 milligrams of ru...

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Bibliographic Details
Main Authors: Robinson, Simon P., Streusand, Virginia J., Chatfield, J. Mark, Portis, Archie R.
Format: Artigo
Language:Inglês
Published: 1988
Subjects:
Metabolism and Enzymology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1055706/
https://ncbi.nlm.nih.gov/pubmed/16666412
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