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Isolation of Viable Wheat Male Gametophytes of Different Stages of Development and Variations in Their Protein Patterns

Procedures have been designed to isolate viable immature male gametophytes from wheat (Triticum aestivum L.) anthers of different stages of development. Maceration of anthers by a micro-blender allowed for the release of numerous intact vacuolate microspores. Tris-buffered media prevented tricellula...

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Detalhes bibliográficos
Main Authors: Vergne, Philippe, Dumas, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1055695/
https://ncbi.nlm.nih.gov/pubmed/16666487
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