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Isolation of Intact Chloroplasts from Dunaliella tertiolecta

Cells of Dunaliella tertiolecta from the log phase of growth were broken by rapid extrusion at low pressure through a Yeda press and the chloroplasts were isolated by centrifugation through a Percoll gradient. Osmolarity of the growth media, the suspending media, and the Percoll gradient was kept id...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Goyal, Arun, Betsche, Thomas, Tolbert, N. E.
Format: Artigo
Langue:Inglês
Publié: 1988
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1055621/
https://ncbi.nlm.nih.gov/pubmed/16666345
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