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Plant Plasma Membrane Proteins : II. Biotinylation of Daucus Carota Protoplasts and Detection of Plasma Membrane Polypeptides after Sds-Page

The ability of two biotinylating reagents, sulfosuccinimidobiotin and sulfosuccinimidyl 2-(biotinamido)ethyl-1,3′-dithiopropionate, to label plasma membrane proteins was examined. These compounds form covalent bonds with the free amino groups of proteins and label the proteins with biotin. Biotinyla...

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Autors principals: Grimes, Howard D., Slay, Raymond M., Hodges, Thomas K.
Format: Artigo
Idioma:Inglês
Publicat: 1988
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1055597/
https://ncbi.nlm.nih.gov/pubmed/16666324
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