In-gel DNA radiolabelling and two-dimensional pulsed field gel electrophoresis procedures suitable for fingerprinting and mapping small eukaryotic genomes
A simple method for complete genome radiolabelling is described, involving long-wave UV exposure of agarose-embedded chromosomal DNA and [α-(32)P]dCTP incorporation mediated by the Klenow fragment. Experiments on the budding yeast genome show that the labelling procedure can be coupled with two new...
Sábháilte in:
| Foilsithe in: | Nucleic Acids Res |
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| Príomhchruthaitheoirí: | , , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe / Cruthaithe: |
Oxford University Press
2000
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| Ábhair: | |
| Rochtain ar líne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105387/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10773096/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.10.e48 |
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