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Diagnostic Potential of Toscana Virus N Protein Expressed in Escherichia coli
The nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coli by using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoass...
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Main Authors: | , , , , , , |
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Format: | Artigo |
Language: | Inglês |
Published: |
American Society for Microbiology (ASM)
1998
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Subjects: | |
Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC105295/ https://ncbi.nlm.nih.gov/pubmed/9774559 |
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