PCR-Enzyme Immunoassay for Detection of Streptococcus pneumoniae DNA in Cerebrospinal Fluid Samples from Patients With Culture-Negative Meningitis

A PCR-based assay was developed to amplify a conserved region of the pneumococcal autolysin gene. The amplified product was labelled with digoxigenin-labelled dUTP and was detected with a biotin-labelled probe in an enzyme immunoassay (EIA). The assay was initially tested with suspensions of various...

Fuld beskrivelse

Bibliografiske detaljer

Udgivet i:	J Clin Microbiol
Principais autores:	Cherian, Thomas, Lalitha, M. K., Manoharan, Anand, Thomas, Kurien, Yolken, Robert H., Steinhoff, Mark C.
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1998
Fag:	Bacteriology
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105248/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9817881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.12.3605-3608.1998
Tags:	Tilføj Tag Ingen Tags, Vær først til at tagge denne postø!