PCR-Enzyme Immunoassay for Detection of Streptococcus pneumoniae DNA in Cerebrospinal Fluid Samples from Patients With Culture-Negative Meningitis

A PCR-based assay was developed to amplify a conserved region of the pneumococcal autolysin gene. The amplified product was labelled with digoxigenin-labelled dUTP and was detected with a biotin-labelled probe in an enzyme immunoassay (EIA). The assay was initially tested with suspensions of various...

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Λεπτομέρειες βιβλιογραφικής εγγραφής

Εκδόθηκε σε:	J Clin Microbiol
Κύριοι συγγραφείς:	Cherian, Thomas, Lalitha, M. K., Manoharan, Anand, Thomas, Kurien, Yolken, Robert H., Steinhoff, Mark C.
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	American Society for Microbiology (ASM) 1998
Θέματα:	Bacteriology
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105248/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9817881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.12.3605-3608.1998
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