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5′ Nuclease PCR Assay To Detect Yersinia pestis

The 5′ nuclease PCR assay uses a fluorescently labeled oligonucleotide probe (TaqMan) to rapidly detect and quantitate DNA templates in clinical samples. We developed a 5′ nuclease PCR assay targeting the plasminogen activator gene (pla) of Yersinia pestis. The assay is species specific, with a dete...

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Main Authors: Higgins, James A., Ezzell, John, Hinnebusch, B. Joseph, Shipley, Michelle, Henchal, Erik A., Ibrahim, M. Sofi
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 1998
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC105032/
https://ncbi.nlm.nih.gov/pubmed/9666006
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