Method for Reduction of Inhibition in a Mycobacterium tuberculosis-Specific Ligase Chain Reaction DNA Amplification Assay

The present study describes the identification of inhibitors of a Mycobacterium tuberculosis-specific gap ligase chain reaction (LCR) DNA amplification assay as well as a method for their removal. A major contributor to inhibition was deduced to be a calcium phosphate precipitate, CaHPO(4). The prec...

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Main Authors: Leckie, Gregor W., Erickson, Dwight D., He, Qizhi, Facey, Ingrid E., Lin, Bor-Chian, Cao, Jianli, Halaka, Folim G.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1998
Teme:
Mycobacteriology and Aerobic Actinomycetes
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC104622/
https://ncbi.nlm.nih.gov/pubmed/9508309
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