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Positive and negative mutant selection in the human histone hairpin-binding protein using the yeast three-hybrid system

We have used the yeast three-hybrid system in a positive selection for mutants of the human histone hairpin-binding protein (HBP) capable of interacting with non-canonical hairpins and in a negative selection for loss-of-binding mutants. Interestingly, all mutations from the positive selection are l...

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Bibliografiske detaljer
Main Authors: Martin, Franck, Michel, Fabrice, Zenklusen, Daniel, Müller, Berndt, Schümperli, Daniel
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2000
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC102788/
https://ncbi.nlm.nih.gov/pubmed/10710426
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