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Real-time monitoring of in vitro transcriptional RNA synthesis using fluorescence resonance energy transfer

We have developed a novel method for real-time monitoring of RNA synthesis in in vitro transcription reactions using fluorescence resonance energy transfer (FRET). Two 15mer DNAs, either of which was labeled with Bodipy493/503 as a donor or Cy5 as an acceptor, were prepared. When the two fluorescent...

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Detaylı Bibliyografya
Asıl Yazarlar: Sei-Iida, Yukari, Koshimoto, Hiroyuki, Kondo, Satoshi, Tsuji, Akihiko
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 2000
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC102744/
https://ncbi.nlm.nih.gov/pubmed/10871382
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