Discriminating duplex and hairpin oligonucleotides using chemical shifts: application to the anticodon stem–loop of Escherichia coli tRNA(Phe)
A sensitive NMR spectroscopic method for detection of duplex forms of self-complementary nucleic acid sequences has been implemented. The G·U wobble base pair formed between a (15)N-labeled strand and an unlabeled probe strand is used to identify the duplex. The guanine imino resonance, with its cha...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2000
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102693/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10908367/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.15.e74 |
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