Substitution of Asp-210 in HAP1 (APE/Ref-1) eliminates endonuclease activity but stabilises substrate binding

HAP1, also known as APE/Ref-1, is the major apurinic/apyrimidinic (AP) endonuclease in human cells. Previous structural studies have suggested a possible role for the Asp-210 residue of HAP1 in the enzymatic function of this enzyme. Here, we demonstrate that substitution of Asp-210 by Asn or Ala eli...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Rothwell, D. G., Hang, B., Gorman, M. A., Freemont, P. S., Singer, B., Hickson, I. D.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2000
Assuntos:	Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871340/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.11.2207
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