Optimised ligation of oligonucleotides by thermal ligases: comparison of Thermus scotoductus and Rhodothermus marinus DNA ligases to other thermophilic ligases

We describe the characterisation of four thermostable NAD(+)-dependent DNA ligases, from Thermus thermophilus (Tth), Thermus scotoductus (Ts), Rhodothermus marinus (Rm) and Thermus aquaticus (Taq), by an assay which measures ligation rate and mismatch discrimination. Complete libraries of octa-, non...

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Bibliographic Details
Main Authors: Housby, J. Nicholas, Thorbjarnardóttir, Sigrídur H., Jónsson, Zophonías O., Southern, Edwin M.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2000
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC102565/
https://ncbi.nlm.nih.gov/pubmed/10637340
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