Generation of Mutant Murine Cytomegalovirus Strains from Overlapping Cosmid and Plasmid Clones

We have developed a cosmid and plasmid system to generate mutant strains of murine cytomegalovirus (MCMV). The system is based on a series of seven overlapping cosmid clones that regenerate MCMV when cotransfected into mouse cells. The unaltered cosmids produce MCMV that is indistinguishable from wi...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Ehsani, Mariam E., Abraha, Tshge W., Netherland-Snell, Cecile, Mueller, Niklaus, Taylor, Meghan M., Holwerda, Barry
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Replication
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102093/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10982341/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.74.19.8972-8979.2000
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