Generation of Mutant Murine Cytomegalovirus Strains from Overlapping Cosmid and Plasmid Clones

We have developed a cosmid and plasmid system to generate mutant strains of murine cytomegalovirus (MCMV). The system is based on a series of seven overlapping cosmid clones that regenerate MCMV when cotransfected into mouse cells. The unaltered cosmids produce MCMV that is indistinguishable from wi...

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Main Authors: Ehsani, Mariam E., Abraha, Tshge W., Netherland-Snell, Cecile, Mueller, Niklaus, Taylor, Meghan M., Holwerda, Barry
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2000
Teme:
Replication
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC102093/
https://ncbi.nlm.nih.gov/pubmed/10982341
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