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Flow Cytometric Detection of Leishmania Parasites in Human Monocyte-Derived Macrophages: Application to Antileishmanial-Drug Testing

A flow cytometric technique was developed for detection of amastigotes of the protozoan Leishmania infantum in human nonadherent monocyte-derived macrophages. The cells were fixed and permeabilized with paraformaldehyde-ethanol, and intracellular amastigotes were labeled with Leishmania lipophosphog...

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Main Authors: Di Giorgio, C., Ridoux, O., Delmas, F., Azas, N., Gasquet, M., Timon-David, P.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2000
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Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC101605/
https://ncbi.nlm.nih.gov/pubmed/11036025
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