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Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for breast cancer studies.

INTRODUCTION: A prerequisite to accurate interpretation of RQ-PCR data is robust data normalization. A commonly used method is to compare the cycle threshold (CT) of target miRNAs with those of a stably expressed endogenous (EC) miRNA(s) from the same sample. Despite the large number of studies repo...

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Main Authors: Ailbhe M McDermott, Michael J Kerin, Nicola Miller
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Acceso en liña:http://europepmc.org/articles/PMC3877087?pdf=render
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