Transphosphorylation of E. coli proteins during production of recombinant protein kinases provides a robust system to characterize kinase specificity

Protein kinase specificity is of fundamental importance to pathway regulation and signal transduction. Here, we report a convenient system to monitor the activity and specificity of recombinant protein kinases expressed in E.coli. We apply this to the study of the cytoplasmic domain of the plant r...

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Bibliographic Details
Main Authors: Xia eWu, Man-Ho eOh, Hyoung Seok Kim, Daniel eSchwartz, Brian S. Imai, Peter M. Yau, Steven D. Clouse, Steven eHuber
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2012-11-01
Series:Frontiers in Plant Science
Subjects:
BAK1
BRI1
FLS2
E.coli transphosphorylation assay
PEPR1
CDPK
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00262/full
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