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Transphosphorylation of E. coli proteins during production of recombinant protein kinases provides a robust system to characterize kinase specificity
Protein kinase specificity is of fundamental importance to pathway regulation and signal transduction. Here, we report a convenient system to monitor the activity and specificity of recombinant protein kinases expressed in E.coli. We apply this to the study of the cytoplasmic domain of the plant r...
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| Główni autorzy: | , , , , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Frontiers Media S.A.
2012-11-01
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| Seria: | Frontiers in Plant Science |
| Hasła przedmiotowe: | |
| Dostęp online: | http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00262/full |
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