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Transphosphorylation of E. coli proteins during production of recombinant protein kinases provides a robust system to characterize kinase specificity

Protein kinase specificity is of fundamental importance to pathway regulation and signal transduction. Here, we report a convenient system to monitor the activity and specificity of recombinant protein kinases expressed in E.coli. We apply this to the study of the cytoplasmic domain of the plant r...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Xia eWu, Man-Ho eOh, Hyoung Seok Kim, Daniel eSchwartz, Brian S. Imai, Peter M. Yau, Steven D. Clouse, Steven eHuber
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Frontiers Media S.A. 2012-11-01
Sarja:Frontiers in Plant Science
Aiheet:
Linkit:http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00262/full
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