Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana

Abstract Clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) based genome editing technology has been developed from the adaptive immune system used by many bacteria and archaea for combating against viruses. Here, we utilize the Cas9 with dual gRNAs de...

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Main Authors: Kangquan Yin, Ting Han, Ke Xie, Jinping Zhao, Junqi Song, Yule Liu
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2019-03-01
Colecção:Phytopathology Research
Assuntos:
Genome editing
Virus resistance
Geminivirus
Acesso em linha:http://link.springer.com/article/10.1186/s42483-019-0017-7
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