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Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana

Abstract Clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) based genome editing technology has been developed from the adaptive immune system used by many bacteria and archaea for combating against viruses. Here, we utilize the Cas9 with dual gRNAs de...

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Auteurs principaux: Kangquan Yin, Ting Han, Ke Xie, Jinping Zhao, Junqi Song, Yule Liu
Format: Artigo
Langue:Inglês
Publié: BMC 2019-03-01
Collection:Phytopathology Research
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Accès en ligne:http://link.springer.com/article/10.1186/s42483-019-0017-7
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