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Engineering an enhanced, thermostable, monomeric bacterial luciferase gene as a reporter in plant protoplasts.
The application of the luxCDABE operon of the bioluminescent bacterium Photorhabdus luminescens as a reporter has been published for bacteria, yeast and mammalian cells. We report here the optimization of fused luxAB (the bacterial luciferase heterodimeric enzyme) expression, quantum yield and its a...
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Main Authors: | , , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Public Library of Science (PLoS)
2014-01-01
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Colecção: | PLoS ONE |
Acesso em linha: | http://europepmc.org/articles/PMC4182741?pdf=render |
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