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Engineering an enhanced, thermostable, monomeric bacterial luciferase gene as a reporter in plant protoplasts.

The application of the luxCDABE operon of the bioluminescent bacterium Photorhabdus luminescens as a reporter has been published for bacteria, yeast and mammalian cells. We report here the optimization of fused luxAB (the bacterial luciferase heterodimeric enzyme) expression, quantum yield and its a...

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Detalhes bibliográficos
Main Authors: Boyu Cui, Lifeng Zhang, Yunhong Song, Jinsong Wei, Changfu Li, Tietao Wang, Yao Wang, Tianyong Zhao, Xihui Shen
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science (PLoS) 2014-01-01
Colecção:PLoS ONE
Acesso em linha:http://europepmc.org/articles/PMC4182741?pdf=render
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