Differentially expressed genes from RNA-Seq and functional enrichment results are affected by the choice of single-end versus paired-end reads and stranded versus non-stranded protocols

Abstract Background RNA-Seq is now widely used as a research tool. Choices must be made whether to use paired-end (PE) or single-end (SE) sequencing, and whether to use strand-specific or non-specific (NS) library preparation kits. To date there has been no analysis of the effect of these choices on...

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Main Authors: Susan M. Corley, Karen L. MacKenzie, Annemiek Beverdam, Louise F. Roddam, Marc R. Wilkins
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2017-05-01
Colecção:BMC Genomics
Assuntos:
RNA-Seq
Transcriptomics
Paired-end reads
Single-end reads
Differential expression
Strand-specific
Acesso em linha:http://link.springer.com/article/10.1186/s12864-017-3797-0
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