The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization

Abstract Engineering of fungal laccases with optimum catalytic activity at alkaline pH has been a long-lasting challenge. In this study, a mutant library containing 3000 clones was obtained by error-prone PCR to adapt the optimum pH of a fungal laccase Lcc9 from the basidiomycete Coprinopsis cinerea...

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Main Authors: Qiang Yin, Gang Zhou, Can Peng, Yinliang Zhang, Ursula Kües, Juanjuan Liu, Yazhong Xiao, Zemin Fang
Formato: Artigo
Idioma:Inglês
Publicado em: SpringerOpen 2019-09-01
Colecção:AMB Express
Assuntos:
Fungal laccase
Directed evolution
Alkaline pH activity
Indigo dye
Decolorization
Acesso em linha:http://link.springer.com/article/10.1186/s13568-019-0878-2
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