Enzymatic Dissociation Induces Transcriptional and Proteotype Bias in Brain Cell Populations

Different cell isolation techniques exist for transcriptomic and proteotype profiling of brain cells. Here, we provide a systematic investigation of the influence of different cell isolation protocols on transcriptional and proteotype profiles in mouse brain tissue by taking into account single-cell...

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Detalhes bibliográficos
Main Authors: Daniele Mattei, Andranik Ivanov, Marc van van Oostrum, Stanislav Pantelyushin, Juliet Richetto, Flavia Mueller, Michal Beffinger, Linda Schellhammer, Johannes vom vom Berg, Bernd Wollscheid, Dieter Beule, Rosa Chiara Paolicelli, Urs Meyer
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2020-10-01
Colecção:International Journal of Molecular Sciences
Assuntos:
microglia
astrocytes
neurons
enzymatic digestion
single-cell sequencing
protocol
Acesso em linha:https://www.mdpi.com/1422-0067/21/21/7944
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