Intracellular flow cytometry complements RT-qPCR detection of circulating SARS-CoV-2 variants of concern

Basic and antiviral research on SARS-CoV-2 rely on cellular assays of virus replication in vitro. In addition, accurate detection of virus-infected cells and released virus particles is needed to study virus replication and to profile new candidate antiviral drugs. Here, by flow cytometry, we detect...

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Bibliographic Details
Main Authors: Emiel Vanhulle, Becky Provinciael, Joren Stroobants, Anita Camps, Piet Maes, Kurt Vermeire
Format: Artigo
Language:Inglês
Published: Future Science Ltd 2022-04-01
Series:BioTechniques
Subjects:
antiviral drug
duplex RT-qPCR
flow cytometry
nucleocapsid
omicron variant
SARS-CoV-2
Online Access:https://www.future-science.com/doi/10.2144/btn-2022-0018
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