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A non-fluorescent HaloTag blocker for improved measurement and visualization of protein synthesis in living cells [version 1; peer review: 2 approved]

Background: HaloTag is a modified bacterial enzyme that binds rapidly and irreversibly to an array of synthetic ligands, including chemical dyes. When expressed in live cells in conjunction with a protein of interest, HaloTag can be used to study protein trafficking, synthesis, and degradation. For...

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Bibliografische gegevens
Hoofdauteurs: Laurie D. Cohen, Ayub Boulos, Noam E. Ziv
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: F1000 Research Ltd 2020-04-01
Reeks:F1000Research
Online toegang:https://f1000research.com/articles/9-302/v1
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