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A unique mono- and diacylglycerol lipase from Penicillium cyclopium: heterologous expression, biochemical characterization and molecular basis for its substrate selectivity.

A cDNA gene encoding a mature peptide of the mono- and diacylglycerol lipase (abbreviated to PcMdl) from Penicillium cyclopium PG37 was cloned and expressed in Pichia pastoris GS115. The recombinant PcMdl (rePcMdl) with an apparent molecular weight of 39 kDa showed the highest activity (40.5 U/mL of...

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書誌詳細
主要な著者: Zhong-Biao Tan, Jian-Fang Li, Xue-Ting Li, Ying Gu, Min-Chen Wu, Jing Wu, Jun-Qing Wang
フォーマット: Artigo
言語:Inglês
出版事項: Public Library of Science (PLoS) 2014-01-01
シリーズ:PLoS ONE
オンライン・アクセス:http://europepmc.org/articles/PMC4106778?pdf=render
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