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Measurement of cerebral oxygen pressure in living mice by two-photon phosphorescence lifetime microscopy

Summary: The ability to quantify partial pressure of oxygen (pO2) is of primary importance for studies of metabolic processes in health and disease. Here, we present a protocol for imaging of oxygen distributions in tissue and vasculature of the cerebral cortex of anesthetized and awake mice. We des...

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Detalhes bibliográficos
Main Authors: Eva Erlebach, Luca Ravotto, Matthias T. Wyss, Jacqueline Condrau, Thomas Troxler, Sergei A. Vinogradov, Bruno Weber
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2022-06-01
Colecção:STAR Protocols
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Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2666166722002507
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