Feasibility of a workflow for the molecular characterization of single cells by next generation sequencing

The purpose of the study was to explore the feasibility of a protocol for the isolation and molecular characterization of single circulating tumor cells (CTCs) from cancer patients using a single-cell next generation sequencing (NGS) approach. To reach this goal we used as a model an artificial samp...

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Detalles Bibliográficos
Autores principales: Francesca Salvianti, Giada Rotunno, Francesca Galardi, Francesca De Luca, Marta Pestrin, Alessandro Maria Vannucchi, Angelo Di Leo, Mario Pazzagli, Pamela Pinzani
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2015-09-01
Colección:Biomolecular Detection and Quantification
Materias:
Single cell
Circulating tumor cells
NGS
Somatic mutations
Microgenomics
Acceso en línea:http://www.sciencedirect.com/science/article/pii/S2214753515300024
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