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Assessing mycoplasma contamination of cell cultures by qPCR using a set of universal primer pairs targeting a 1.5 kb fragment of 16S rRNA genes.
Mycoplasmas (a generic name for Mollicutes) are a predominant bacterial contaminant of cell culture and cell derived products including viruses. This prokaryote class is characterized by very small size and lack of a cell wall. Consequently, mycoplasmas escape ultrafiltration and visualization under...
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Main Authors: | , , , , , |
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格式: | Artigo |
語言: | Inglês |
出版: |
Public Library of Science (PLoS)
2017-01-01
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叢編: | PLoS ONE |
在線閱讀: | http://europepmc.org/articles/PMC5321415?pdf=render |
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