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Influence of primer & probe chemistry and amplification target on reverse transcription digital PCR quantification of viral RNA

Compared to other PCR technologies, digital PCR is a potentially highly accurate approach for the quantification of nucleic acid fragments. This study describes the impact of four experimental factors, namely primer and probe chemistry, PCR amplification target, duplexing, and template type, on the...

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Detalhes bibliográficos
Main Authors: Fran Van Heuverswyn, Maria Karczmarczyk, Heinz Schimmel, Stefanie Trapmann, Hendrik Emons
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2016-09-01
Colecção:Biomolecular Detection and Quantification
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Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2214753516300201
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