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Influence of primer & probe chemistry and amplification target on reverse transcription digital PCR quantification of viral RNA
Compared to other PCR technologies, digital PCR is a potentially highly accurate approach for the quantification of nucleic acid fragments. This study describes the impact of four experimental factors, namely primer and probe chemistry, PCR amplification target, duplexing, and template type, on the...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Elsevier
2016-09-01
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Colecção: | Biomolecular Detection and Quantification |
Assuntos: | |
Acesso em linha: | http://www.sciencedirect.com/science/article/pii/S2214753516300201 |
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