Screening of lectins from South American plants used as affinity ligands to purify rhEPO

Two groups of isoforms of rhEPO, at a concentration of 300 µg/ml, were tested as putative inhibitors of the lectinic hemagglutination reaction in order to obtain affinity ligand(s) for hormone purification: groups I (pI: 3.80; 3.89; 3.95; 4.07, 4.15 and 4.26) and groups II (pI: 4.15, 4.26; 4.38; 4.5...

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Главные авторы: G.I. Amadeo, R. Moreira, R. Lima, D. Teixeira, R. Kratje, M. Etcheverrigaray
Формат: Artigo
Язык:Inglês
Опубликовано: Brazilian Society of Chemical Engineering 2003-03-01
Серии:Brazilian Journal of Chemical Engineering
Предметы:
rhEPO
lectin affinity purification
isoform selection
Online-ссылка:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322003000100005
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