Addressing bias in small RNA library preparation for sequencing: a new protocol recovers microRNAs that evade capture by current methods

Recent advances in sequencing technology have helped unveil the unexpected complexity and diversity of small RNAs. A critical step in small RNA library preparation for sequencing is the ligation of adapter sequences to both the 5’ and 3’ ends of small RNAs. Studies have shown that adapter ligation i...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Jeanette eBaran-Gale, C. Lisa eKurtz, Michael eErdos, Christina eSison, Alice eYoung, Emily E Fannin, Peter S Chines, Praveen eSethupathy
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2015-12-01
Schriftenreihe:Frontiers in Genetics
Schlagworte:
microRNA
Sequencing
Small RNA library preparation
Adapter ligation bias
Adapter dimers
Online Zugang:http://journal.frontiersin.org/Journal/10.3389/fgene.2015.00352/full
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