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Selection and validation of endogenous reference genes for qRT-PCR analysis in leafy spurge (Euphorbia esula).
Quantitative real-time polymerase chain reaction (qRT-PCR) is the most important tool in measuring levels of gene expression due to its accuracy, specificity, and sensitivity. However, the accuracy of qRT-PCR analysis strongly depends on transcript normalization using stably expressed reference gene...
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Main Authors: | , , , , |
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格式: | Artigo |
語言: | Inglês |
出版: |
Public Library of Science (PLoS)
2012-01-01
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叢編: | PLoS ONE |
在線閱讀: | http://europepmc.org/articles/PMC3419244?pdf=render |
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