Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures

Extracellular vesicles (EVs) can be harvested from cell culture supernatants and from all body fluids. EVs can be conceptually classified based on their size and biogenesis as exosomes and microvesicles. Nowadays, it is however commonly accepted in the field that there is a much higher degree of het...

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Main Authors: Oscar P. B. Wiklander, R. Beklem Bostancioglu, Joshua A. Welsh, Antje M. Zickler, Florian Murke, Giulia Corso, Ulrika Felldin, Daniel W. Hagey, Björn Evertsson, Xiu-Ming Liang, Manuela O. Gustafsson, Dara K. Mohammad, Constanze Wiek, Helmut Hanenberg, Michel Bremer, Dhanu Gupta, Mikael Björnstedt, Bernd Giebel, Joel Z. Nordin, Jennifer C. Jones, Samir EL Andaloussi, André Görgens
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2018-06-01
Colecção:Frontiers in Immunology
Assuntos:
exosomes
microvesicles
extracellular vesicles
extracellular vesicle flow cytometry
bead-based flow cytometry
exosome analysis
Acesso em linha:https://www.frontiersin.org/article/10.3389/fimmu.2018.01326/full
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