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Accurately Determining Esterase Activity via the Isosbestic Point of p-Nitrophenol

Esterase is an important enzyme for ester hydrolysis or synthesis. Its activity, however, has not been accurately ascertained due to a lack of accurate protocols. In this study, the isosbestic point of p-nitrophenol was found and used as the marker for its activity. The methodology avoided decomposi...

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Detalhes bibliográficos
Main Authors: Yangyang Peng, Shiyu Fu, Hao Liu, Lucian A. Lucia
Formato: Artigo
Idioma:Inglês
Publicado em: North Carolina State University 2016-10-01
Colecção:BioResources
Assuntos:
Acesso em linha:http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_4_10099_Peng_Esterase_Activity_Nitrophenol_9
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