Comparative Evaluation of a Novel Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) Assay, LAMP, Conventional PCR, and Leaf-Disc Baiting Methods for Detection of Phytophthora sojae

Early and accurate detection of the causal pathogen Phytophthora sojae is crucial for effective prevention and control of root and stem rot and seedling damping-off of soybean. In the present study, a novel isothermal amplification assay was developed for detecting P. sojae. This 25 min assay includ...

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Principais autores: Tingting Dai, Xiao Yang, Tao Hu, Binbin Jiao, Yue Xu, Xiaobo Zheng, Danyu Shen
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019-08-01
coleção:Frontiers in Microbiology
Assuntos:
oomycetes
plant destroyers
field diagnosis
Phytophthora sansomeana
Phytophthora melonis
Phytophthora vignae
Acesso em linha:https://www.frontiersin.org/article/10.3389/fmicb.2019.01884/full
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