It Takes Two to Tango: Combining Conventional Culture With Molecular Diagnostics Enhances Accuracy of Streptococcus pneumoniae Detection and Pneumococcal Serogroup/Serotype Determination in Carriage

BackgroundThe specificity of molecular methods for the detection of Streptococcus pneumoniae carriage is under debate. We propose a procedure for carriage surveillance and vaccine impact studies that increases the accuracy of molecular detection of live pneumococci in polymicrobial respiratory sampl...

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Main Authors: Willem R. Miellet, Janieke van Veldhuizen, David Litt, Rob Mariman, Alienke J. Wijmenga-Monsuur, Paul Badoux, Tessa Nieuwenhuijsen, Rebecca Thombre, Sanaa Mayet, Seyi Eletu, Carmen Sheppard, Marianne Alice van Houten, Nynke Y. Rots, Elizabeth Miller, Norman K. Fry, Elisabeth A. M. Sanders, Krzysztof Trzciński
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2022-04-01
Colecção:Frontiers in Microbiology
Assuntos:
Streptococcus pneumoniae (pneumococcus)
carriage
diagnostic accuracy
qPCR (quantitative PCR)
conventional culture
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fmicb.2022.859736/full
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