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Quantitative measurement of brightness from living cells in the presence of photodepletion.

The brightness of fluorescently labeled proteins provides an excellent marker for identifying protein interactions in living cells. Quantitative interpretation of brightness, however, hinges on a detailed understanding of the processes that affect the signal fluctuation of the fluorescent label. Her...

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Autores principales: Kwang-Ho Hur, Patrick J Macdonald, Serkan Berk, C Isaac Angert, Yan Chen, Joachim D Mueller
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science (PLoS) 2014-01-01
Colección:PLoS ONE
Acceso en línea:http://europepmc.org/articles/PMC4018325?pdf=render
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