Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections

Summary: Photo-isolation chemistry (PIC) enables isolation of transcriptome information from locally defined areas by photo-irradiation. Here, we present an optimized PIC protocol for formalin-fixed frozen and paraffin mouse sections and fresh-frozen mouse sections. We describe tissue section prepar...

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Detalhes bibliográficos
Main Authors: Mizuki Honda, Ryuichi Kimura, Akihito Harada, Kazumitsu Maehara, Kaori Tanaka, Yasuyuki Ohkawa, Shinya Oki
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2022-06-01
Colecção:STAR Protocols
Assuntos:
Gene Expression
Molecular Biology
Molecular/Chemical Probes
RNAseq
Sequence analysis
Sequencing
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S266616672200226X
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