Optofluidic Single-Cell Genome Amplification of Sub-micron Bacteria in the Ocean Subsurface

Optofluidic single-cell genome amplification was used to obtain genome sequences from sub-micron cells collected from the euphotic and mesopelagic zones of the northwestern Sargasso Sea. Plankton cells were visually selected and manually sorted with an optical trap, yielding 20 partial genome sequen...

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Main Authors: Zachary C. Landry, Kevin Vergin, Christopher Mannenbach, Stephen Block, Qiao Yang, Paul Blainey, Craig Carlson, Stephen Giovannoni
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2018-06-01
Serija:Frontiers in Microbiology
Teme:
single-cell genomics
E01-9C-26
marine microbiology
microfluidics
optofluidics
bermuda-atlantic time-series
Online dostop:https://www.frontiersin.org/article/10.3389/fmicb.2018.01152/full
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