Optimized RNA Extraction and Northern Hybridization in Streptomycetes

<p>Abstract</p> <p>Northern blot hybridization is a useful tool for analyzing transcript patterns. To get a picture of what really occurs in vivo, it is necessary to use a protocol allowing full protection of the RNA integrity and recovery and unbiased transfer of the entire transc...

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Bibliographic Details
Main Authors: Tagliavia Marcello, Taravella Anna, Marineo Sandra, Puglia Anna, La Farina Mario
Format: Artigo
Language:Inglês
Published: BMC 2010-03-01
Series:Biological Procedures Online
Subjects:
streptomycetes
total RNA purification
RNA processing
RNA degradation
RNA glyoxylation
alkaline blotting
northern hybridization
Online Access:http://www.biologicalproceduresonline.com/content/12/1/9027
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