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Common contaminants in next-generation sequencing that hinder discovery of low-abundance microbes.

Unbiased high-throughput sequencing of whole metagenome shotgun DNA libraries is a promising new approach to identifying microbes in clinical specimens, which, unlike other techniques, is not limited to known sequences. Unlike most sequencing applications, it is highly sensitive to laboratory contam...

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Detalhes bibliográficos
Main Authors: Martin Laurence, Christos Hatzis, Douglas E Brash
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science (PLoS) 2014-01-01
Colecção:PLoS ONE
Acesso em linha:http://europepmc.org/articles/PMC4023998?pdf=render
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