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Molecular engineering of the salicylate-inducible transcription factor Sal7AR for orthogonal and high gene expression in Escherichia coli.
I have previously identified a metagenomic fragment (~4 kb) containing the salicylate (2-hydroxybenzoate)-responsive transcriptional regulator Sal7AR. Taking advantage of the inert nature of salicylate to common genetic switches used in Escherichia coli, here I developed a salicylate-inducible high...
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Formato: | Artigo |
Idioma: | Inglês |
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Public Library of Science (PLoS)
2018-01-01
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Series: | PLoS ONE |
Acceso en liña: | http://europepmc.org/articles/PMC5894983?pdf=render |
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