Robustness of Next Generation Sequencing on Older Formalin-Fixed Paraffin-Embedded Tissue.

Next Generation Sequencing (NGS) technologies are used to detect somatic mutations in tumors and study germ line variation. Most NGS studies use DNA isolated from whole blood or fresh frozen tissue. However, formalin-fixed paraffin-embedded (FFPE) tissues are one of the most widely available clinica...

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Main Authors: Danielle Mercatante Carrick, Michele G Mehaffey, Michael C Sachs, Sean Altekruse, Corinne Camalier, Rodrigo Chuaqui, Wendy Cozen, Biswajit Das, Brenda Y Hernandez, Chih-Jian Lih, Charles F Lynch, Hala Makhlouf, Paul McGregor, Lisa M McShane, JoyAnn Phillips Rohan, William D Walsh, Paul M Williams, Elizabeth M Gillanders, Leah E Mechanic, Sheri D Schully
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science (PLoS) 2015-01-01
Colecção:PLoS ONE
Acesso em linha:http://europepmc.org/articles/PMC4519244?pdf=render
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