GC/MS-based 13C metabolic flux analysis resolves the parallel and cyclic photomixotrophic metabolism of Synechocystis sp. PCC 6803 and selected deletion mutants including the Entner-Doudoroff and phosphoketolase pathways

Abstract Background Cyanobacteria receive huge interest as green catalysts. While exploiting energy from sunlight, they co-utilize sugar and CO2. This photomixotrophic mode enables fast growth and high cell densities, opening perspectives for sustainable biomanufacturing. The model cyanobacterium Sy...

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Main Authors: Dennis Schulze, Michael Kohlstedt, Judith Becker, Edern Cahoreau, Lindsay Peyriga, Alexander Makowka, Sarah Hildebrandt, Kirstin Gutekunst, Jean-Charles Portais, Christoph Wittmann
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2022-04-01
Colecção:Microbial Cell Factories
Assuntos:
13C metabolic flux analysis
Cyanobacteria
GC–MS
NMR
Glycolytic shunt
Oxidative pentose phosphate pathway
Acesso em linha:https://doi.org/10.1186/s12934-022-01790-9
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