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Development and Optimization of Expression, Purification, and ATPase Assay of KaiC for Medium-Throughput Screening of Circadian Clock Mutants in Cyanobacteria

The slow but temperature-insensitive adenosine triphosphate (ATP) hydrolysis reaction in KaiC is considered as one of the factors determining the temperature-compensated period length of the cyanobacterial circadian clock system. Structural units responsible for this low but temperature-compensated...

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Main Authors: Dongyan Ouyang, Yoshihiko Furuike, Atsushi Mukaiyama, Kumiko Ito-Miwa, Takao Kondo, Shuji Akiyama
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2019-06-01
Colecção:International Journal of Molecular Sciences
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Acesso em linha:https://www.mdpi.com/1422-0067/20/11/2789
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