Two-Round Treatment With Propidium Monoazide Completely Inhibits the Detection of Dead Campylobacter spp. Cells by Quantitative PCR

Campylobacter spp. are known as important foodborne gastroenteric pathogens worldwide. Campylobacter spp. can exist in a viable but non-culturable (VBNC) state under unsuitable environmental conditions, which is undetectable by conventional culture methods. Quantitative polymerase chain reaction (qP...

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Main Authors: Ayaka Okada, Mizuki Tsuchida, Md. Matiur Rahman, Yasuo Inoshima
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2022-04-01
Colecção:Frontiers in Microbiology
Assuntos:
Campylobacter
culture method
propidium monoazide
quantitative PCR
viable but non-culturable
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fmicb.2022.801961/full
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