Development of an enzyme immunoassay for poliovirus antigens

An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal co...

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Bibliographic Details
Main Authors: Newton Hashimoto, Fabrício José Benati, Flávio Lauretti, Rosa Elisa Carvalho Linhares, Carlos Mitihiko Nozawa
Format: Artigo
Language:Inglês
Published: Instituto de Tecnologia do Paraná (Tecpar) 2007-01-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Enzyme immunoassay
Poliovirus antigen
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005
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